Expression Optimization
Routine testing of multiple expression/induction conditions to optimize protein expression
Analysis by SDS-PAGE and Western Blot of crude, soluble and pull down fractions
Protein Purification
Pilot to bulk scale
Endogenous and recombinant protein production
Protein refolding from inclusion bodies
Biochemical and biophysical assay grade purity
Protease cleavage of fusion and purification tags
Affinity tag and enzymatic modifications for assay development
15N, 13C, 2H (D) labeling for structural determination by NMR
Quality Control/ Analytics
SDS-PAGE
Analytical SEC
Static light scattering
Western blot
Activity Assays
Mass spectrometry